The serpin family of serine proteinase inhibitors control the key cascades of the blood including coagulation, complement activation, fibrinolysis and, in the case of α-1-antitrypsin, protect lung tissue against proteolytic attack. Antithrombin-III is the first line of defense against thrombosis. Its conformational derivative, latent antithrombin, is an anti-angiogenic agent with its plasma levels giving an index of antithrombin turnover and instability.
Canterbury Scientific Ltd has long experience in the preparation and purification of serpins through its Senior Scientists, Dr.s Maurice Owen and Peter Elliott, supported by colleagues from the Christchurch and University of Cambridge groups - Stephen Brennan, Aiwu Zhou and Paul Harper - who are now consultant advisors to Canterbury Scientific. We provide a range of clinically significant serpins for analytical and research purposes in both native and recombinant forms and welcome enquiries about further products.
Native Plasma Serpins
- α-1-Antitrypsin (PiMM)
- Antithrombin-III
- Latent Antithrombin-III
Normal purified human antithrombin supplied in a lyophilized form.
Latent antithrombin [relaxed, 6-stranded, hyperstable, lyophilized] prepared and isolated as in Blood [1999] 94, 3388-96 & J Biol Chem [2003] 278, 15116-22.
Recombinant Serpins
Supplied frozen in buffer at an appropriate pH for long-term storage. They are prepared, isolated and purified to standards of crystallisation as described in the stated references.
- rα-1-Antitrypsin Pittsburgh (Met358Arg) Human α-1-antitrypsin with its reactive centre methionine replaced by arginine to allow thrombin specific inhibition or cleavage. (J Biol Chem [2003] 278:15116-22).
- rPAI-1 (Human, Wild-type or Stabilised Active Form) Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). Human PAI-1, prepared as in Structure [1999] 7:111-18, or in a form stabilised by mutations (Berkenpas et al EMBO J. 14: 2969-77).
- rTBG Human thyroxine-binding globulin prepared and isolated as in PNAS [2006]13321-26.
- rCBG (Rat or Mouse) Human corticosteroid-binding globulin gives poor yields on recombinant expression as compared to rat or mouse CBG which have a close identity in structure and function.
- rSMB (Human Somatomedin B) Somatomedin B is the domain of vitronectin that binds to PAI-1, cell surface receptors and integrins. Crystallised in Nat Struct Biol [2003] 10:541-4 and prepared as in Protein Sci [2007] 16:1502–8.
- rAngiotensinogen These species-variable angiotensinogens are the source in the circulation of the vasopressor angiotensin peptides. Recombinantly prepared to crystallisation standards.
- rHuman Angiotensinogen
- rMouse Angiotensinogen
- rRat Angiotensinogen
Electrophoretic Phenotyping
These are supplied in a lyophilized form and are intended as phenotyping controls- α-1-Antitrypsin MM
- α-1-Antitrypsin MZ
- α-1-Antitrypsin MS
Please contact us for details.
14 Pope Street
Christchurch
New Zealand
ph: +64 3 343 3345
fax: +64 3 343 3342
